The Effects of Acute Glucoprivation on Adrenomedullary Function in SHR and WKY Rats

We have shown previously, acute intraperitoneal administration of 2-deoxy-d-glucose (2DG) into Sprague-Dawley rats led to activation the adrenal medulla chromaffin cells, indicated with increased protein kinase activity and tyrosine hydroxylase (TH) phosphorylation, as well plasma adrenaline glucose levels. Here we used spontaneous hypertensive (SHR) Wistar Kyoto (WKY) investigate whether hypertension alters basal cell function, or response these cells 2DG treatment. At level, found no differences in TH protein, catecholamine levels between SHR WKY despite a significant difference level systolic blood pressure; nor were there (BG). Furthermore, vehicle animals evoked changes any parameter measured SHR, but increases pSer19TH, BG WKY. Single episode glucoprivation PKA CDK/MAPK, pSer40TH, pSer31TH, activity, addition PKC, CAMKII, pSer19TH These findings are which indicates does not impact function gland. It also appears that alter glucoprivation. The showed greater kinases phosphorylation saline when compared possible reasons for further discussed.